Electrochemical studies of ion-channel behavior of annexin V in phosphatidylcholine bilayer membranes

The ion-channel behavior of annexin V in phosphatidylcholine bilayer membranes (BLM) is studied from the peaks currents of Fe(CN)63-/4- and Ru(bpy)32+ which are used as electrochemical probes. It is demonstrated that annexin V can form pores in the lipid membrane that allow both Fe(CN)63-/4- and Ru(bpy)32+ probes to reach the surface of the substrate electrode. The influence of Ca2+, pH and protein concentration on the ion-channel formation is also examined. This may provide a simple and general way to study this important biological process.