کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
181821 | 459411 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ultra-sensitive cholesterol biosensor based on low-temperature grown ZnO nanoparticles Ultra-sensitive cholesterol biosensor based on low-temperature grown ZnO nanoparticles](/preview/png/181821.png)
A high-sensitive cholesterol amperometric biosensor based on the immobilization of cholesterol oxidase (ChOx) onto the ZnO nanoparticles has been fabricated which shows a very high and reproducible sensitivity of 23.7 μA mM−1 cm−2, detection limit (based on S/N ratio) 0.37 ± 0.02 nM, response time less than 5 s, linear range from 1.0 to 500.0 nM and correlation coefficient of R = 0.9975. A relatively low value of enzyme’s kinetic parameter (Michaelis–Menten constant) ∼4.7 mM has been obtained which indicates the enhanced enzymatic affinity of ChOx to Cholesterol. To the best of our knowledge, this is the first report in which such a very high-sensitivity and low detection limit has been achieved for the cholesterol biosensor by using ZnO nanostructures modified electrodes.
Journal: Electrochemistry Communications - Volume 11, Issue 1, January 2009, Pages 118–121