کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
182218 459420 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantum-dots based electrochemical immunoassay of interleukin-1α
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Quantum-dots based electrochemical immunoassay of interleukin-1α
چکیده انگلیسی

We describe a quantum-dot (QD, CdSe@ZnS) based electrochemical immunoassay to detect a protein biomarker, interleukin-1α (IL-1α). QD conjugated with anti-IL-1α antibody was used as a label in an immunorecognition event. After a complete sandwich immunoreaction among the primary IL-1α antibody (immobilized on the avidin-modified magnetic beads), IL-1α, and the QD-labeled secondary antibody, QD labels were attached to the magnetic-bead surface through the antibody-antigen immunocomplex. Electrochemical stripping analysis of the captured QDs was used to quantify the concentration of IL-1α after an acid-dissolution step. The streptavidin-modified magnetic beads and the magnetic separation platform were used to integrate a facile antibody immobilization (through a biotin/streptavidin interaction) with immunoreactions and the isolation of immunocomplexes from reaction solutions in the assay. The voltammetric response is highly linear over the range of 0.5–50 ng ml−1 IL-1α, and the limit of detection is estimated to be 0.3 ng ml−1 (18 pM). This QD-based electrochemical immunoassay shows great promise for rapid, simple, and cost-effective analysis of protein biomarkers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 9, Issue 7, July 2007, Pages 1573–1577
نویسندگان
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