Electrochemical studies of novel chitosan/TiO2 bioactive electrode for biosensing application

Bioactive electrode of uniformly dispersed TiO2 in Chitoan (CS) was fabricated on ITO substrate for immobilization of horseradish peroxidase (HRP). Enhanced surface porosity and decrease in relative proportion of carbonyl functionality of CS in CS/TiO2 matrix was observed. Current voltage characteristic of CS/TiO2 matrix was enhanced by a factor of four possibly due to covalent and hydrogen binding of Ti atoms with hydroxyl and amino groups of CS. The immobilization of HRP on CS/TiO2 had increased resistance for charge transfer. This is possibly due to strongly binding of HRP with CS/TiO2 matrix and controlling transport of the ions of the supporting electrolyte.