کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1862511 | 1530651 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two-compartment model for competitive hybridization on molecular biochips
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
فیزیک و نجوم (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Two-compartment model for competitive hybridization on molecular biochips Two-compartment model for competitive hybridization on molecular biochips](/preview/png/1862511.png)
چکیده انگلیسی
During competitive hybridization the specific and non-specific fractions of tested biomolecules in solution bind jointly with the specific probes immobilized in a separate cell of a microchip. The application of two-compartment model to the two-component hybridization allows analytically investigating the underlying kinetics. It is shown that the behaviour with the non-monotonous growth of complexes formed by the non-specific fraction on a probe cell is a typical feature of competitive hybridization for both diffusion-limited and reaction-limited kinetics. The physical reason behind such an evolution consists in the fact that the characteristic hybridization time for the perfect complexes turns out longer with respect to that for the mismatch complexes. This behaviour should be taken into account for the choice of optimum hybridization and washing conditions for the analysis of specific fraction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physics Letters A - Volume 360, Issues 4â5, 8 January 2007, Pages 491-494
Journal: Physics Letters A - Volume 360, Issues 4â5, 8 January 2007, Pages 491-494
نویسندگان
V.R. Chechetkin,