Preparation of Amperometric Glucose Biosensor Based on 4-Mercaptobenzoic Acid

A novel glucose biosensor was fabricated by a combination of a self-assembled monolayer (SAM) of 4-mercaptobenzoic acid and the Langmuir-Blodgett (LB) technique. Because of the catalysis of Prussian Blue contained in the LB film layers, the prepared amperometric biosensor worked at a very low potential range around 0.0 V vs. Ag/AgCl. The optimum operating conditions for glucose biosensor were investigated by varying the glucose oxidase immobilization time, the applied potential and the pH of buffer solution. The steady-state current responses of the glucose biosensor showed a good linear relationship to glucose concentrations from 0.1 mM to 154 mM.