کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1884357 1043339 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Qγ−H2AX, an analysis method for partial-body radiation exposure using γ-H2AX in non-human primate lymphocytes
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Qγ−H2AX, an analysis method for partial-body radiation exposure using γ-H2AX in non-human primate lymphocytes
چکیده انگلیسی

We previously used the γ-H2AX assay as a biodosimeter for total-body irradiation (TBI) exposure (γ-rays) in a rhesus macaque (Macaca mulatta) model. Utilizing peripheral blood lymphocytes and plucked hairs, we obtained statistically significant γ-H2AX responses days after total-body exposure to 1–8.5 Gy (60Co γ-rays at 55 cGy min−1). Here, we introduce a partial-body exposure analysis method, Qγ−H2AX, which is based on the number of γ-H2AX foci per damaged cells as evident by having one or more γ-H2AX foci per cell. Results from the rhesus monkey – TBI study were used to establish Qγ−H2AX dose-response calibration curves to assess acute partial-body exposures. γ-H2AX foci were detected in plucked hairs for several days after in vivo irradiation demonstrating this assay’s utility for dose assessment in various body regions. The quantitation of γ-H2AX may provide a robust biodosimeter for analyzing partial-body exposures to ionizing radiation in humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Measurements - Volume 46, Issue 9, September 2011, Pages 877–881
نویسندگان
, , , , , ,