کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923332 | 1048885 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dynamic subcellular localization of estrogen receptor alpha during the first two cleavages of mouse preimplantation embryos
ترجمه فارسی عنوان
محلی سازی سلول های پلاسمایی از گیرنده استروژن آلفا در طی دو مرحله اول از جنین های قبل از تکامل موش
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Zygotic gene activation (ZGA) is one of the most important events after mouse fertilization, but the mechanisms underpinning it are still unclear. Estrogen receptor alpha (ERα) is a versatile player in animal development. Our preliminary studies showed that ERα-specific antagonists blocked mouse 2-cell development and inhibited ZGA related gene expression, indicating an indispensable role of maternal ERα in early mouse preimplantation embryo development (PED). Here, we performed immunostaining detection to investigate the cell cycle specific subcellular localization of ERα, and serine 118 phosphorylated ERα (pERα-S118), during the first two cleavages of mouse PED. Our results showed that ERα nuclear localization appeared at 1-cell S-phase, disappeared at metaphase, and reappeared since the G1 phase of 2-cell embryos. Nuclear expression of pERα-S118 started at the 1-cell S-phase, but was absent at the G1 phase of 2-cell embryos, and reappeared since the 2-cell S-phase. Interestingly, pERα-S118 showed a dynamic expression pattern among nuclear, nuclear surface, cytoplasm and cell membrane. These results indicate that maternal derived ERα might still function in the first two cleavages of mouse PED, during which ZGA occurs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 118, Issue 3, April 2016, Pages 317-321
Journal: Acta Histochemica - Volume 118, Issue 3, April 2016, Pages 317-321
نویسندگان
Songhua Xu, Xiuli Lian, Xiaoxiang Cheng, Chanchan Song, Lin He, Yue Liu, Junming Chen, Juan Du, Lili Pang, Shie Wang,