کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923709 | 1048908 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Signal amplification in immunohistochemistry: Loose-jointed deformable heteropolymeric HRP conjugates vs. linear polymer backbone HRP conjugates
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Improvements in reagents and protocols for immunohistochemistry have led to increased sensitivity of detection systems. A significant level of signal amplification was achieved by the chain-polymer conjugate technology utilizing enzyme-labeled inert “backbone” molecule of dextran (Dako). However, the relatively large size of the dextran molecule in aqueous phase appears to create spatial hindrance compromising the penetrative ability of the detection reagent. Novel AmpliStain⢠detection systems (SDT GmbH, Baesweiler, Germany) seem to overcome these constraints offering a more compact and deformable conjugate design that facilitates agile penetration through the narrowest diffusion pathways in tissue sections. Here, we compared the level of signal amplification achievable with AmpliStainâ¢-HRP (SDT) and EnVisionâ¢+-HRP (Dako). Our results show that the AmpliStainâ¢-HRP systems allow higher dilutions of primary antibodies in both immunohistochemistry and ELISA. Compared with EnVisionâ¢+, anti-mouse AmpliStain⢠enables at least three times more sensitive detection of mouse antibodies, whereas anti-rabbit AmpliStain⢠is ten times more sensitive than anti-rabbit EnVisionâ¢+.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 115, Issue 6, July 2013, Pages 587-594
Journal: Acta Histochemica - Volume 115, Issue 6, July 2013, Pages 587-594
نویسندگان
Igor Buchwalow, Werner Boecker, Eduard Wolf, Vera Samoilova, Markus Tiemann,