کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923856 | 1048918 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of anti-phospholipid antibodies on a human trophoblast cell line (HTR-8/SVneo) Effects of anti-phospholipid antibodies on a human trophoblast cell line (HTR-8/SVneo)](/preview/png/1923856.png)
SummaryAntibodies to phospholipids (aPL) have been shown to adversely affect trophoblast invasion in vivo and in vitro. HTR-8/SVneo cells derived from first trimester of pregnancy extravillous trophoblast were studied. Matrigel invasion assay, cytochemistry and cell-based enzyme-linked immunosorbant assay (ELISA) with aPL or normal IgG was used. Our data show that aPL at 100 μg/ml decrease invasiveness of HTR-8/SVneo cells to 60% of control (p<0.01), and this was also shown for primary cytotrophoblast (to 15.5% of control, p<0.001). aPL treatment caused a significant decrease in integrin α1, α5, and β1 proteins (86%, 84%, and 87%, respectively). We conclude that HTR-8/SVneo cell culture is a suitable model to study mechanisms of action of aPL on trophoblast, which in HTR-8/SVneo cells inhibit invasion by decreasing integrins α5, α1, and β1.
Journal: Acta Histochemica - Volume 112, Issue 1, January 2010, Pages 34–41