Mitochondria-rich cells in amphibian skin epithelium: Relationship of immuno- and peanut lectin labeling pattern and transport functions

Mitochondria-rich cells are an integral component of the epidermis of amphibian skin and play a functional role. Whereas the principal cell compartment of the epithelium is specialized almost exclusively for active uptake of sodium, the mitochondria-rich cells perform other diverse ion-transport functions, including transport of Cl−, H+, HCO3− and organic molecules. These transporting functions differ in different species. Antibodies, such as those directed against band 3, H+-ATPase, and also peanut lectin (PNA), bind specifically to the mitochondria-rich cells, but do so differently in various species. Examination of these immunolocalizations and lectin labeling in the skin of over 10 amphibian species, including both Anurans and Urodeles, illustrate species-specific differences. The binding pattern and the transport capabilities of the skin in the various species do not show a universal correlation, they appear to be species specific and do not permit construction of a general scheme common to all the species studied. The mitochondria-rich cells of heterocellular epithelia and their roles in ion transport remain a subject that requires further studies to elucidate their particular functions within the framework of the whole epithelium.