کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1924108 1048937 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rad10-YFP focus induction in response to UV depends on RAD14 in yeast
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Rad10-YFP focus induction in response to UV depends on RAD14 in yeast
چکیده انگلیسی

Rad14 is a DNA damage recognition protein in yeast Nucleotide Excision Repair (NER) and believed to function early in the cascade of events. The function of Rad14 presumably precedes that of the Rad1–Rad10 endonuclease complex, which functions in a downstream step incising DNA 5′ to the site of DNA damage. We investigated whether recruitment of Rad10 to UV-induced DNA damage sites in live cells is dependent on Rad14 using fluorescence microscopy. Experiments were carried out using Saccharomyces cerevisiae strains in which the gene for Rad14 was fused to Cyan Fluorescent Protein (Rad14-CFP) and that of Rad10 was fused to Yellow Fluorescent Protein (Rad10-YFP). Rad14-CFP forms nuclear localized CFP fluorescent foci in response to UV irradiation with the peak induction occurring 15 min post-irradiation. In contrast, Rad10-YFP foci form in response to UV with the peak induction occurring 2 h post-irradiation. Recruitment of Rad14-CFP is not dependent on the RAD10 gene but Rad10-YFP is recruited to UV-induced YFP foci in a RAD14-dependent fashion. Time-lapse experiments indicate that Rad14-CFP foci are transient, typically persisting less than 6 min. Together these data support the model that yeast NER protein assembly is step-wise whereas Rad14 required to recruit Rad10 and Rad14 involvement is transient.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 113, Issue 4, July 2011, Pages 409–415
نویسندگان
, , , , , ,