کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941613 1536901 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer
ترجمه فارسی عنوان
Adipocytes تکثیر سلول های سرطانی پانکراس را از طریق انتقال گلوتامین ارتقا می دهند
کلمات کلیدی
FCS، سرم جنین گوساله؛ GPNA، اسید l-گلوتامیک، γ-p-نیترانیلید-هیدروکلراید؛ گلن، گلوتامین؛ GSI، مهار کننده سنتتاز گلوتامین؛ GLS، گلوتامیناز؛ GDH، گلوتامات دهيدروژناز؛ GS، glutamine synthetase؛ پانین، نئوپلازی داخل پاتولوژیک پانکراس؛ PDAC
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Adipocytes promote pancreatic cancer cell proliferation in nutrient-poor conditions.
• Adipocytes secrete glutamine.
• Adipocytes rescue pancreatic cancer cell proliferation in the absence of glutamine.
• Glutamine transfer may underlie adipocyte-induced pancreatic cancer cell proliferation.

Adipocytes promote progression of multiple cancers, but their role in pancreatic intraepithelial neoplasia (PanIN) and ductal adenocarcinoma (PDAC) is poorly defined. Nutrient transfer is a mechanism underlying stromal cell-cancer crosstalk. We studied the role of adipocytes in regulating in vitro PanIN and PDAC cell proliferation with a focus on glutamine metabolism. Murine 3T3L1 adipocytes were used to model adipocytes. Cell lines derived from PKCY mice were used to model PanIN and PDAC. Co-culture was used to study the effect of adipocytes on PanIN and PDAC cell proliferation in response to manipulation of glutamine metabolism. Glutamine secretion was measured with a bioanalyzer. Western blotting was used to study the effect of PanIN and PDAC cells on expression of glutamine-related enzymes in adipocytes. Adipocytes promote proliferation of PanIN and PDAC cells, an effect that was amplified in nutrient-poor conditions. Adipocytes secrete glutamine and rescue PanIN and PDAC cell proliferation in the absence of glutamine, an effect that was glutamine synthetase-dependent and involved PDAC cell-induced down-regulation of glutaminase expression in adipocytes. These findings suggest glutamine transfer as a potential mechanism underlying adipocyte-induced PanIN and PDAC cell proliferation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 7, September 2016, Pages 144–149
نویسندگان
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