کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941635 1536901 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elasto-mechanical properties of living cells
ترجمه فارسی عنوان
خواص الاستو ـ مکانیکی سلول های زنده
کلمات کلیدی
میکروسکوپ نیروی اتمی؛ AFM؛ مدول یانگ؛ سلول اندوتلیال؛ نوسان شبه تناوبی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Hours long time dependent elasticity measurements were performed on living cells.
• Quasi-periodical oscillation of the elastic modulus was detected with AFM.
• FFT and correlation factor calculation prove the existence of these oscillations.

The possibility to directly measure the elasticity of living cell has emerged only in the last few decades. In the present study the elastic properties of two cell lines were followed. Both types are widely used as cell barrier models (e.g. blood-brain barrier). During time resolved measurement of the living cell elasticity a continuous quasi-periodic oscillation of the elastic modulus was observed. Fast Fourier transformation of the signals revealed that a very limited number of three to five Fourier terms fitted the signal in the case of human cerebral endothelial cells. In the case of canine kidney epithelial cells more than 8 Fourier terms did not result a good fit. Calculating the correlation between nucleus and periphery of the signals revealed a higher correlation factor for the endothelial cells compared to the epithelial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 7, September 2016, Pages 303–308
نویسندگان
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