کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941636 1536901 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Creation of mouse TNFR2-selective agonistic TNF mutants using a phage display technique
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Creation of mouse TNFR2-selective agonistic TNF mutants using a phage display technique
چکیده انگلیسی


• We generated a TNF mutant with full TNFR2-selective agonist activity.
• This mutant was identified using a phage display technique.
• This agonist exhibited high binding selectivity and bioactivity to mouse TNFR2.
• This would be a powerful tool to elucidate the functional roles of mouse TNFR2.

Tumor necrosis factor-α (TNF), which is an immuno-modulatory cytokine, has been suggested to cause inflammatory responses as well as protection against tissue dysfunction by binding two types of TNF receptor (TNFR1/TNFR2). However, the physiological effects of TNFR2-specific activation remain unclear. We therefore aimed to generate a TNF mutant with full TNFR2-selective agonist activity as a functional analytical tool. In this study, we utilized a phage display technique to create mouse TNFR2 (mTNFR2)-selective TNF mutants that bind specifically to mTNFR2 and show full bioactivity compared with wild-type TNF. A new phage library displaying TNF mutants was created, in which nine amino acid residues at the predicted receptor-binding site were randomized. From this library, an agonistic TNF mutant exhibiting high binding selectivity and bioactivity to mTNFR2 was isolated. We propose that this TNF mutant would be a powerful tool with which to elucidate the functional roles of mTNFR2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 7, September 2016, Pages 309–315
نویسندگان
, , , , , , , , ,