کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941643 1536901 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species
ترجمه فارسی عنوان
فعال سازی ایزوآنزیم پروتئین کیناز C معمولی و جدید بوسیله گونه های مولکولی diacylglycerol مختلف
کلمات کلیدی
cPKC، پروتئین کیناز معمولی C؛ DG، diacylglycerol؛ DGK، کیناز diacylglycerol؛ MBP، میلین قطعه پروتئین اساسی 4/14؛ nPKC، پروتئین کیناز جدید C؛ PKC، پروتئین کیناز C؛ TPA، 12-O-Tetradecanoylphorbol-13-acetateProtein kinase C؛ Isoform؛ دیسیلگ
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• We comprehensively analyzed activation of c/nPKC isozymes by different DG species.
• c/nPKCs have different sensitivities and dependences on DG.
• c/nPKCs have a distinct preference for different fatty acid-containing DG species.
• This differential regulation would be important for PKCs' physiological functions.

A variety of diacylglycerol (DG) molecular species are produced in stimulated cells. Conventional (α, βII and γ) and novel (δ, ε, η and θ) protein kinase C (PKC) isoforms are known to be activated by DG. However, a comprehensive analysis has not been performed. In this study, we analyzed activation of the PKC isozymes in the presence of 2–2000 mmol% 16:0/16:0-, 16:0/18:1-, 18:1/18:1-, 18:0/20:4- or 18:0/22:6-DG species. PKCα activity was strongly increased by DG and exhibited less of a preference for 18:0/22:6-DG at 2 mmol%. PKCβII activity was moderately increased by DG and did not have significant preference for DG species. PKCγ activity was moderately increased by DG and exhibited a moderate preference for 18:0/22:6-DG at 2 mmol%. PKCδ activity was moderately increased by DG and exhibited a preference for 18:0/22:6-DG at 20 and 200 mmol%. PKCε activity moderately increased by DG and showed a moderate preference for 18:0/22:6-DG at 2000 mmol%. PKCη was not markedly activated by DG. PKCθ activity was the most strongly increased by DG and exhibited a preference for 18:0/22:6-DG at 2 and 20 mmol% DG. These results indicate that conventional and novel PKCs have different sensitivities and dependences on DG and a distinct preference for shorter and saturated fatty acid-containing and longer and polyunsaturated fatty acid-containing DG species, respectively. This differential regulation would be important for their physiological functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 7, September 2016, Pages 361–366
نویسندگان
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