کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941667 1536902 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Different characteristics between menadione and menadione sodium bisulfite as redox mediator in yeast cell suspension
ترجمه فارسی عنوان
ویژگی های مختلف بین منادیون و بیسولفیت سدیم منادیون به عنوان واسطه بازتوانی در تعلیق سلول مخمر
کلمات کلیدی
منادیون؛ منادیون بیسولفیت سدیم؛ انتقال الکترون؛ واسطه ریدوکس؛ سلول های مخمری
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Menadione promoted the production of active oxygen species.
• MSB kept more stable increase in electron transfer from yeast cells to Fe(CN)63- than menadione.
• Menadione promoted oxidation of NAD(P)H much faster than MSB in permeabilized cells.
• Menadione had much stronger inhibitory effect on the proliferation of yeast cells than MSB.
• Inhibitory effect of menadione on the proliferation was reduced by superoxide dismutase and catalase.

Menadione promoted the production of active oxygen species (AOS) in both yeast cell suspension and the crude enzymes from the cells, but menadione sodium bisulfite (MSB) had little effect on the production of AOS in the cell suspension. MSB kept the stable increase in the electron transfer from intact yeast cells to anode compared to menadione, but the electron transfer promoted by MSB was inhibited in permeabilized yeast cell suspension. Menadione promoted oxidation of NAD(P)H much faster than MSB in permeabilized yeast cell suspension, suggesting the oxidative stress due to consumption of NAD(P)H. The proliferation of yeast cells was inhibited by menadione under aerobic conditions rather than anaerobic conditions, and the inhibitory effect was reduced by superoxide dismutase and catalase. The effect of MSB on the proliferation was much smaller than that of menadione. The above facts suggest that harmless MSB promotes the electron transfer from plasma membrane of yeast cells to anode. On the other hand, harmful menadione might promote the electron transfer from cytosol and plasma membrane to anode and dissolved oxygen.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 6, July 2016, Pages 88–93
نویسندگان
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