کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1941701 | 1536903 | 2016 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Thioredoxin 1 regulation of protein S-desulfhydration Thioredoxin 1 regulation of protein S-desulfhydration](/preview/png/1941701.png)
• Thioredoxin 1 facilitates protein S-desulfhydration.
• Thioredoxin 1 directly interacts with S-sulfhydrated proteins.
• Cysteine-32 in Thioredoxin 1 is required for protein S-desulfhydration.
The importance of H2S in biology and medicine has been widely recognized in recent years, and protein S-sulfhydration is proposed to mediate the direct actions of H2S bioactivity in the body. Thioredoxin 1 (Trx1) is an important reducing enzyme that cleaves disulfides in proteins and acts as an S-denitrosylase. The regulation of Trx1 on protein S-sulfhydration is unclear. Here we showed that Trx1 facilitates protein S-desulfhydration. Overexpression of Trx1 attenuated the basal level and H2S-induced protein S-sulfhydration by direct interaction with S-sulfhydrated proteins, i.e., glyceraldehyde 3-phosphate dehydrogenase and pyruvate carboxylase. In contrast, knockdown of Trx1 mRNA expression by short interfering RNA or blockage of Trx1 redox activity with PX12 or 2,4-dinitrochlorobenzene enhanced protein S-sulfhydration. Mutation of cysteine-32 but not cysteine-35 in the Trp–Cys32–Gly–Pro–Cys35 motif eliminated the binding of Trx1 with S-sulfhydrated proteins and abolished the S-desulfhydrating effect of Trx1. All these data suggest that Trx1 acts as an S-desulfhydrase.
Journal: Biochemistry and Biophysics Reports - Volume 5, March 2016, Pages 27–34