Identification of a 48 kDa form of unconventional myosin 1c in blood serum of patients with autoimmune diseases

• Protein markers linked with autoimmune diseases have searched in blood serum.
• 46 kDa fragment of human unconventional myosin Ic isoform b (46/Myc 1c) was identified.
• High level of 46/Myc 1c in blood serum of MS and RA patients was detected.

We searched for protein markers present in blood serum of multiple sclerosis (MS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients in comparison to healthy human individuals. We used precipitation/extraction methods and MALDI TOF/TOF mass spectrometry, and identified a protein with Mr ~46 kDa as a fragment of human unconventional myosin IC isoform b (Myo1C). Western blotting with specific anti-human Myo1C antibodies confirmed the identity. Screening of blood serum samples from different autoimmune patients for the presence of Myo1c revealed its high level in MS and RA patients, relatively low level in SLE patients, and undetected in healthy donors. These data are suggesting that the level of p46 Myo1C in blood serum is a potential marker for testing of autoimmune diseases.

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