کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941852 1536907 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aquaporin10 is a pseudogene in cattle and their relatives
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Aquaporin10 is a pseudogene in cattle and their relatives
چکیده انگلیسی


• AQP10, mainly expressed in the GI tract, has turned to a pseudogene in the cow.
• The other ruminantia such as sheep and goat also have the AQP10 pseudogene.
• The presence of authentic AQP10 in the pig may indicate that AQP10 turned to a pseudogene 65 million years ago.

BackgroundAlthough AQP10 is mainly expressed in the human GI tract, its physiological role is unclear. In fact, we previously reported that mouse AQP10 is a pseudogene. It is possible that AQP10 is also a pseudogene in other animals.MethodsGenome databases were searched for AQP10 orthologs and the genomic DNA of each candidate pseudogene was sequenced to confirm its mutations. The expression of the AQP10 mRNA was examined by RT-PCR in the small intestine where human AQP10 is highly expressed.ResultsThe genomic database of some mammals had insertions and deletions in the exons of the AQP10 gene, including cattle (Bos taurus), sheep (Ovis aries) and goats (Capra hircus). In the bovine AQP10 gene, exon 1 and 5 had deletions resulting in a frame-shift or a premature termination, respectively, which were confirmed by the direct exon sequencing of the genomic DNA. In the RT-PCR experiments, the PCR primer sets for exon 1/2 and exon 4/5 failed to detect the bands for AQP10 mRNA in the duodenum and jejunum. Similar AQP10 gene mutations were also confirmed in the genomic DNA from sheep and goats. Although these animals were derived from porcine ancestors, the exons of the swine (Sus scrofa) AQP10 gene were complete without mutations. Therefore, AQP10 gene might have turned to a pseudogene around 65 million years before when cattle evolved from porcine ancestors.ConclusionAQP10 of ruminantia which regurgitate and rechew their food may have lost its role possibly due to the redundant expression of other aquaglyceroporins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 1, May 2015, Pages 16–21
نویسندگان
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