کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1972897 | 1060299 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell volume regulation following hypotonic shock in hepatocytes isolated from Sparus aurata
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The response of isolated hepatocytes of Sparus aurata to hypotonic shock was studied by the aid of videometric and light scattering methods. The isolated cells exposed to a rapid change (from 370 to 260Â mOsm/kg) of the osmolarity of the bathing solution swelled but thereafter underwent a decrease of cell volume tending to recovery the original size. This homeostatic response RVD (regulatory volume decrease) was inhibited in the absence of extracellular Ca2+ and in the presence of TMB8, an inhibitor of Ca2+ release from intracellular stores. It is likely that Ca2+ entry through verapamil sensitive Ca2+-channels, probably leading to a release of Ca2+ from intracellular stores, is responsible for RVD since the blocker impaired the ability of the cell to recover its volume after the hypotonic shock. RVD tests performed in the presence of various inhibitors of different transport mechanisms, such as BaCl2, quinine, glybenclamide and bumetanide as well as in the presence of a KCl activator, NEM, led us to suggest that the recovery of cell volume in hypotonic solution is accomplished by an efflux of K+ and Clâ through conductive pathways paralleled by the operation of the KCl cotransport, followed by an obliged water efflux from the cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 158, Issue 1, January 2011, Pages 143-149
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 158, Issue 1, January 2011, Pages 143-149
نویسندگان
Caterina Faggio, Agata Torre, Elisa Pelle, Federica Raffa, Valentina Villari, Francesca Trischitta,