کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1973033 | 1060306 | 2011 | 7 صفحه PDF | دانلود رایگان |
Glucose metabolism in free-swimming fasted and fed seabass was studied using deuterated water (2H2O). After transfer to seawater enriched with 4.9% 2H2O for 6-h or for 72-h, positional and mole percent enrichment (MPE) of plasma glucose and water were quantified by 2H NMR and ESI-MS/MS. Plasma water 2H-enrichment reached that of seawater within 6 h. In both fasted and fed fish, plasma glucose MPE increased asymptotically attaining ~ 55% of plasma water enrichment by 72 h. The distribution of 2H-enrichment between the different glucose positions was relatively uniform. The gluconeogenic contribution to glucose that was synthesized during 2H2O administration was estimated from the ratio of position 5 and 2 glucose enrichments. For both fed and fasted fish, gluconeogenesis accounted for 98 ± 1% of the glucose that was produced during the 72-h 2H2O administration period. For fasted fish, gluconeogenic contributions measured after 6 h were identical to 72-h values (94 ± 3%). For fed fish, the apparent gluconeogenic contribution at 6-h was significantly lower compared to 72-h (79 ± 5% versus 98 ± 1%, p < 0.05). This may reflect a brief augmentation of gluconeogenic flux by glycogenolysis after feeding and/or selective enrichment of plasma glucose position 2 via futile glucose-glucose-6-phosphate cycling.
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 160, Issue 3, November 2011, Pages 341–347