کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1973388 1060315 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short-term overcrowding of Atlantic cod, Gadus morhua: Effects on serum-mediated antibacterial activity and transcription of glucose transport and antioxidant defense related genes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Short-term overcrowding of Atlantic cod, Gadus morhua: Effects on serum-mediated antibacterial activity and transcription of glucose transport and antioxidant defense related genes
چکیده انگلیسی

Serum-mediated control of Listonella anguillarum and transcriptional profiles of selected glucose transport and antioxidant defense genes, following short-term overcrowding in Atlantic cod, Gadus morhua were determined. Fish were subjected to overcrowding by reducing the water level in the tank for 1 h and this was repeated thrice over a 12 h period. Blood samples were collected before overcrowding (initial group) and at 2, 24 and 72 h post-crowding. The sera from fish obtained at 2 h post-crowding caused a significant reduction in L. anguillarum counts compared to the initial samples. There was a transcriptional upregulation of the glucose transport-4 and glyceraldehyde-3-phosphate dehydrogenase genes at 2 h after crowding. Gene transcripts of the antioxidant enzymes, Cu/Zn superoxide dismutase (Cu/Zn SOD), catalase and phospholipid hydroperoxide glutathione peroxidase also significantly increased at 2 h post-crowding, but thereafter they returned to their pre-crowding levels with the exception of Cu/Zn SOD that remained significantly higher than the initial group until 72 h. Thus, short-term overcrowding of Atlantic cod leads to a transient enhancement of in vitro serum antibacterial activity and enhanced transcriptional activity of glucose transport and antioxidant defense genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 151, Issue 4, December 2008, Pages 560–565
نویسندگان
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