کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1973407 | 1060315 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vitro effects of noradrenaline on Sydney rock oyster (Saccostrea glomerata) hemocytes In vitro effects of noradrenaline on Sydney rock oyster (Saccostrea glomerata) hemocytes](/preview/png/1973407.png)
Our prior work has shown that the catecholamine hormone, noradrenaline, mediates environmental stress responses in Sydney rock oysters, resulting in impaired immunological function. In the current study, we tested the cellular basis of this stress response. Hemocytes were exposed to noradrenaline in vitro before cell morphology and viability were analyzed. Noradrenaline was shown to induce apoptotic markers, including the loss of mitochondrial membrane potential, DNA fragmentation and plasma membrane blebbing. F-actin appeared to play an important role in the changes observed in hemocytes, being concentrated mostly in the plasma membrane blebs of noradrenaline-treated hemocytes. This may explain why hemocyte adhesion and pseudopodia formation were inhibited by noradrenaline. Cellular dysfunction induced by norarenaline mainly affected the hyalinocyte sub-population of hemocytes, whilst the other major cell type, granulocytes, remained unaffected. Given that hyalinocytes are important immunological effectors, the results of this study help to explain why immunosuppression accompanies noradrenaline-mediated stress responses in oysters.
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 151, Issue 4, December 2008, Pages 691–697