کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1973479 1060318 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Factors altering the haemolytic power of crude venom from Aiptasia mutabilis (Anthozoa) nematocysts
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Factors altering the haemolytic power of crude venom from Aiptasia mutabilis (Anthozoa) nematocysts
چکیده انگلیسی

The effect of different agents upon the haemolytic power of Aiptasia mutabilis crude venom was studied in human erythrocytes to determine its toxicity and stability. Nematocysts were isolated from acontia of the Anthozoan A. mutabilis and submitted to sonication for extracting crude venom. Aliquots of venom were tested in 0.05% erythrocyte suspensions in the presence of various factors such as proteases (papain, collagenase, trypsin, α-chymotrypsin); cations (Ca2+, Mg2+, Ba2+, K+ and Cu2+), osmotic protectants as polyethylenglycole (PEG) of different MW and antioxidant compounds (GSH, cysteine and ascorbic acid). Results demonstrate the dose–response of the haemolytic effect of A. mutabilis. Haemolysis by the crude venom was prevented by Ca2+, Ba2+ and Cu2+ treatment, and to a minor extent by Mg2+ and K+. Papain and PEG with a molecular mass exceeding 1000 Da also prevented haemolysis. These findings are consistent with a pore-forming mechanism of crude venom in erythrocytes rather than an oxidative damage at the employed doses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 152, Issue 3, March 2009, Pages 418–422
نویسندگان
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