کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1977097 1539273 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sodium fluoride induces hypercalcemia resulting from the upregulation of both osteoblastic and osteoclastic activities in goldfish, Carassius auratus
ترجمه فارسی عنوان
فلورید سدیم ناشی از هیپراکلسمی ناشی از ارتقاء فعالیت های استئوبلاستیک و استئوکلاستیک در ماهی قرمز، Carassius auratus
کلمات کلیدی
ALP، آلکالن فسفاتاز؛ CTSK، کتپسین K؛ EF1α، ضریب اصطکاک 1α؛ MMP-9، ماتریکس متالوپروتئیناز-9؛ NaF، فلوراید سدیم؛ NFATc1، عامل هسته ای T-cell فعال و سیتوپلاسمی 1؛ TRAF6، عامل گیرنده TNF 6؛ TRAP، tartrate-resis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

The influence of sodium fluoride (NaF) on calcium metabolism was examined in goldfish (fresh water teleost). At 2 days after administration of NaF (500 ng/g body weight; 5 μg/g body weight) (around 10− 5 to 10− 4 M in goldfish), we indicated that plasma calcium levels upregulated in both doses of NaF-treated goldfish. To examine the mechanism of hypercalcemia by NaF treatments, therefore, direct effects of NaF on osteoblasts and osteoclasts in goldfish were investigated by an original assay system using teleost scale which has osteoblasts, osteoclasts and bone matrix. Alkaline phosphatase activity in the scales increased with the treatment of NaF (10− 6 and 10− 5 M) during 6 h of incubation. Also, tartrate-resistant acid phosphatase activity increased after exposure to NaF (10− 5 M) at the 6 h of incubation. To investigate the osteoclastic activation, the mRNA expression of osteoclastogenesis related factors were examined. The receptor activator of the nuclear factor-κB ligand (RANKL) which is known as a factor for osteoclastogenesis, increased in the NaF-treated scales after 6 h of incubation. The ratio of RANKL/osteoprotegerin (osteoclastogenesis inhibitory factor) significantly increased after 6 h of incubation. Resulting from the increase of RANKL mRNA level, the expression of transcription-regulating factors was significantly increased. Furthermore, the expression of functional genes, cathepsin K and matrix metalloproteinase-9 mRNA, was significantly increased. In our knowledge, this is the first report concerning the effects of NaF on osteoblasts and osteoclasts in teleosts. We concluded that NaF influences calcium metabolism via osteoclastic activation in goldfish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 189, November 2016, Pages 54–60
نویسندگان
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