کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2006014 | 1541721 | 2014 | 9 صفحه PDF | دانلود رایگان |

• Changes of different lipid concentrations in fat bodies of larvae and pupae of Zophobas atratus after injection of Tenmo-AKH were investigated.
• Lipids were analyzed 24 h and 48 h after injection by GC–MS.
• Dose of Tenmo-AKH induces mobility of free fatty acids and other free lipid components.
• AKH influences on carboxylic acids metabolism and their incorporation to major lipids reserves in the fat body.
In insects, neuropeptide adipokinetic hormone (AKH) released from the corpora cardiaca mobilizes lipids and carbohydrates in the fat body. We examined the developmental differences in the action of Tenmo-AKH, a bioanalogue belonging to the adipokinetic/hypertrahelosemic family (AKH/HrTH), on the lipid composition of larval and pupal fat bodies in the beetle Zophobas atratus. Tenmo-AKH was administered to the beetle larvae and pupae either as a single dose or as two doses of 20 pmol during a 24 h interval. Extracts of fat bodies were used to analyse the lipid composition by gas chromatography (GC) combined with mass spectrometry (GC–MS). Control extracts were analyzed using the same method. Fatty acids (FA) and fatty acid methyl esters (FAME) were the most abundant compounds in the fat bodies from both developmental stages. We observed significant differences in their concentrations following hormonal treatment. Tenmo-AKH also induced a distinct increase in larval sterols, fatty alcohols and benzoic acid.
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Journal: Peptides - Volume 58, August 2014, Pages 65–73