Molecular cloning of two distinct precursor genes of NdWFamide, a d-tryptophan-containing neuropeptide of the sea hare, Aplysia kurodai

► NdWFamide is a d-Trp-containing cardioexcitatory tripeptide of Aplysia. ► We have cloned two distinct precursor genes of the peptide. ► Both of the predicted precursor (NWF90/NWF87) encoded a single copy of NdWFamide. ► Expression of NWF90 gene is dominant over NWF87 gene in the abdominal ganglia.