Antioxidant and free radical-scavenging activity of the extracellular death factor in Escherichia coli

The extracellular death factor (EDF) is a newly discovered linear pentapeptide with the sequence of H-Asn-Asn-Trp-Asn-Asn-OH (NNWNN). It could act as a quorum-sensing molecule and participate in the mazEF-mediated cell death in Escherichia coli. In the present study, we firstly studied the antioxidant and free radical-scavenging activities of EDF. EDF could scavenge hydroxyl radicals in vitro and protect protein, lipid and DNA from being damaged by hydroxyl radicals. Our results indicated that this extracellular death factor might have dual effects during the programmed cell death process of Escherichia coli.

Research highlights▶ The antioxidant and free radical-scavenging activities of EDF were firstly studied. ▶ EDF could prevent proteins, lipids and DNA from being damaged by hydroxyl radicals. ▶ EDF might have dual effects during the programmed cell death process of Escherichia coli.