کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2007872 1066388 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species
چکیده انگلیسی

An antifungal peptide with a molecular mass of 9412 and an N-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable Brassica campestris. The purification protocol entailed ion exchange chromatography on Q-Sepharose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on a Superdex peptide column. The antifungal peptide was adsorbed on Affi-gel blue gel and Mono S. It inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC50 value of 8.3 μM and 4.5 μM, respectively. It exhibited dose-dependent binding to lyso-α-lauroyl phosphatidylcholine. The present findings constitute the first report on a non-specific lipid transfer protein from the seeds of a Brassica species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 28, Issue 8, August 2007, Pages 1514–1519
نویسندگان
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