کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2009509 | 1066670 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Transcriptional characteristics of gene expression in the midgut of domestic silkworms (Bombyx mori) exposed to phoxim Transcriptional characteristics of gene expression in the midgut of domestic silkworms (Bombyx mori) exposed to phoxim](/preview/png/2009509.png)
Silkworm (Bombyx mori) is not only an economically important insect but also a model system for lepidoptera. As a vital organ of digestion and nutrient absorption, the midgut of insects also serves as the first physiological barrier to chemical pesticides. In this study, microarray was performed to profile the gene expression changes in the midgut of silkworms exposed to phoxim. After 24 h of phoxim exposure (4.0 μg/mL), 266 genes displayed at least 2.0-fold changes in expression levels. Among them, 192 genes were up-regulated, and 74 genes were down-regulated. The most significant changes were 14.88-fold up-regulation and 23.36-fold down-regulation. According to gene ontology annotation and pathway analysis, differentially expressed genes were mainly classified into different groups based on their potential involvements in detoxification, immunne response, stress response, energy metabolism and transport. Particularly, the transcription levels of detoxification-related genes were up-regulated, such as cytochrome P450s, esterases and glutathione-S-transferase (GST), indicating increased detoxification activity in the midgut. Our study provides new insights into the molecular mechanism of pesticide metabolism in the midgut of insects, which may promote the development of highly efficient insecticides.
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► We present an overview of gene expression at transcription level in the midgut of silkworms exposed to phoxim.
► 266 Genes exhibited at least 2.0-fold changes, with 192 being up-regulated and 74 being down-regulated.
► Gene ontology annotation and pathway analysis were performed to reveal the potential involvements in different processes.
Journal: Pesticide Biochemistry and Physiology - Volume 105, Issue 1, January 2013, Pages 36–43