کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2009649 1066679 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning, characterization and mRNA expression of a ryanodine receptor gene from diamondback moth, Plutella xylostella
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Molecular cloning, characterization and mRNA expression of a ryanodine receptor gene from diamondback moth, Plutella xylostella
چکیده انگلیسی

Diamondback moth, Plutella xylostella (L.), is one of the most destructive insect pests of cruciferous vegetables around the world. Diamide insecticides provide a new option for control of P. xylostella populations resistant to other chemicals. Insect ryanodine receptors are the target sites of the diamide insecticides. The full-length cDNA of a ryanodine receptor gene (PxRyR) was cloned and characterized from P. xylostella. The cDNAs of PxRyR contain a 15,495-bp open reading frame, 267-bp 5′ untranslated region (UTR) and a 3′-UTR of 351-bp. The predicted mature protein consists of 5164 amino acids with a predicted molecular weight of 583.7-kDa. PxRyR shares common structural features with known RyRs: the well-conserved COOH-terminal domain, which forms a functional Ca2+ channel, and a large hydrophilic NH2-terminal domain. PxRyR shows a high level of amino acid sequence identity (78–80%) to the insect RyR isoforms. Ten deletion polymorphism sites were detected in PxRyR cDNAs, suggesting a single PxRyR can produce many polymorphic transcripts. Although the highest mRNA expression level was observed in larva and the lowest in pupa, there was a relatively stable expression during the developmental period from egg to adult. The relative mRNA expression levels of PxRyR were similar among the head, thorax, and abdomen of the fourth-instar larva body. These results can serve as an important basis for the functional expression of PxRyR and for investigating the involvement of target site gene mutations in resistance to the diamide insecticides in P. xylostella.

Figure optionsDownload as PowerPoint slideHighlights
► A ryanodine receptor gene (PxRyR) was cloned from Plutella xylostella.
► PxRyR shows a high level of amino acid sequence identity (78–80%) to insect RyRs.
► Ten deletion polymorphism sites were detected in PxRyR cDNAs.
► The highest mRNA expression was observed in larva and the lowest in pupa.
► mRNA expression was similar among the head, thorax, and abdomen of the larva.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 102, Issue 3, March 2012, Pages 204–212
نویسندگان
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