Molecular diagnostic for detecting the cytochrome b G143S - QoI resistance mutation in Cercospora beticola

► PASA-PCR/gel electrophoresis detects G143S QoI resistance mutation with limited sensitivity. ► Real Time PCR assay effectively detecting 1:10,000 resistant (S143):sensitive (G143) genotypes. ► No significant proportion of the 143S resistant allele was detected in field isolates from Greece.