کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2009975 | 1066701 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glyphosate-induced metabolic changes in susceptible and glyphosate-resistant soybean (Glycine max L.) roots
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Glyphosate (N-(phosphonomethyl)glycine) blocks the shikimate pathway, reducing the biosynthesis of aromatic amino acids, followed by the arrest interruption of protein production and a general metabolic disruption of the phenylpropanoid pathway. Glyphosate-resistance is conferred to soybean by incorporating a gene encoding a glyphosate-insensitive enzyme (CP4-EPSP synthase) that acts in the shikimate pathway. This paper evaluates the metabolic effects caused by this herbicide on the shikimate (shikimate dehydrogenase activity and shikimate content) and phenylpropanoid (phenylalanine ammonia-lyase activity, phenolic and lignin contents) pathways in BRS-133 (susceptible) and BRS-245RR (resistant) soybean (Glycine max L.) roots. In general, the results showed that in susceptible roots (1) glyphosate affects the shikimate pathway (massive shikimate accumulation and enhanced shikimate dehydrogenase activity) and the phenylpropanoid pathway (increase in PAL activity, production of benzoate derivatives and decrease of lignin) and (2) the metabolic disruption contributes to the production of p-hydroxybenzoate and vanillate, which likely originate from shikimate and/or cinnamate and their derivatives. No such changes were observed in the genetically modified soybean consistent with its resistance to glyphosate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 93, Issue 1, January 2009, Pages 28-33
Journal: Pesticide Biochemistry and Physiology - Volume 93, Issue 1, January 2009, Pages 28-33
نویسندگان
Rogério Marchiosi, Maria de Lourdes Lucio Ferrarese, Edicléia Aparecida Bonini, Natália Gomes Fernandes, Ana Paula Ferro, Osvaldo Ferrarese-Filho,