کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2009992 | 1066702 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Oligochitosan was applied by spraying it on tobacco leaves for inhibition of tobacco mosaic virus (TMV). The maximum inhibition of TMV by oligochitosan was observed when inoculation occurred at 24 h after spraying 50 μg mlâ1 oligochitosan. The production of H2O2 and NO in epidermal tobacco cells induced by oligochitosan was investigated by epidermal strip bioassay and LSCM, using cell permeable fluorophore diaminofluorescein diacetate (DAF-2D) and 2â²,7â²-dichlorofluorescin diacetate (H2DCF-DA), respectively. Epidermal tobacco cells treated with oligochitosan resulted in a strong increase of intracellular NO and H2O2. Oligochitosan and NO donor sodium nitroprusside (SNP) induced the defense reaction against tobacco mosaic virus (TMV), and increased phenylalanine ammonia-lyase (PAL) activity. Co-treatment of the tobacco cells with oligochitosan and NO scavenger CPTIO blocked the inducing resistance. The results indicated that the defense response induced by oligochitosan was connected with NO pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 87, Issue 1, January 2007, Pages 78-84
Journal: Pesticide Biochemistry and Physiology - Volume 87, Issue 1, January 2007, Pages 78-84
نویسندگان
Xiaoming Zhao, Xiaoping She, Yuguang Du, Xinmiao Liang,