کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2010049 1066708 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of the cDNA encoding prophenoloxidase-2 (PPO2) and its expression in diamondback moth Plutella xylostella
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Molecular characterization of the cDNA encoding prophenoloxidase-2 (PPO2) and its expression in diamondback moth Plutella xylostella
چکیده انگلیسی
Phenoloxidase (PO) is a key enzyme in the development process of insects. The cDNA of Plutella xylostella prophenoloxidase-2 (PxPPO2) was cloned by means of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA consisted of 2546 bp, containing an open reading frame (ORF) of 2091 bp which encoding 696 amino acids. BLASTp search and neighbor-joining analysis showed that the deduced amino acid sequence had a high identity to the published sequence of PPO2 from other lepidopterous insects, ranging from 72.2% to 78.3%. Semi-quantitative RT-PCR and real-time PCR indicated that the highest amount of PxPPO2 transcripts in eggs or the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae, and pupa. Real-time PCR analysis showed that when the 3rd instar larvae treated with quercetin or kojic acid, the transcript levels of PPO2 became significantly decreased as compared with control ones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 98, Issue 2, October 2010, Pages 158-167
نویسندگان
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