کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2010195 1066721 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets
چکیده انگلیسی

The mechanism of action of oligochitosan, which has shown great antifungal activity against Phytophthora capsiciin vitro, was studied using 2-aminoacridone-labeled oligochitosan (2-AMAC-oligochitosan) and a gel-retardation experiment. Internalization of 2-AMAC-oligochitosan in cysts, germtubes and sporangia of P. capsici was confirmed by confocal laser scan microscopy (CLSM), and the degree of uptake depended on the incubation concentration. 2-AMAC-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. Mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in P. capsici. The gel-retardation experiment showed that oligochitosan bound strongly to DNA and RNA of P. capsici. These results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as DNA and RNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 88, Issue 2, June 2007, Pages 167–175
نویسندگان
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