کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2010241 1066726 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel immobilized cholinesterase for on-site screening of organophosphate and carbamate compounds
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
A novel immobilized cholinesterase for on-site screening of organophosphate and carbamate compounds
چکیده انگلیسی

Acetylcholinesterase was physically adsorbed on polyvinylpyrrolidone K30 to prepare a novel immobilized cholinesterase, which was described for the first time. The immobilized enzyme was tested for its reactivity, enzyme activity stability, the solubility, the influence of pH and buffer, and characteristic reactivity with inhibitors, e.g., organophosphate and carbamate compounds. The result revealed that the immobilized cholinesterase held its original activity in solution, stable enough, easy to dissolve for convenient operation, and this immobilized cholinesterase could be well used for rapid, on-site screening of organophosphate and carbamate compounds, together with a portable pesticide analyzer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 86, Issue 3, November 2006, Pages 162–166
نویسندگان
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