کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2010246 | 1066726 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved in vitro rearing system for the human head louse allows the determination of resistance to formulated pediculicides
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
An improved in vitro rearing system, based on a silicone-reinforced Parafilm® M membrane, human hair tufts and reconstituted human blood, enabled the large-scale rearing of pediculicide-susceptible (EC-HL) and resistant (SF-HL and BR-HL) strains of human head lice. Developmental time differed for early instars but differences became synchronized as lice matured. Mean survivorship amongst the three strains reared in vitro were not significantly different when compared to EC-HL and SF-HL reared in vivo. The efficacies of three pediculicidal products were assessed under semi-clinical conditions using a modified version of the in vitro rearing system. Treatments of 1% permethrin in acetone, Nix®, Rid® or Pronto® Plus to hair tufts following manufacturer's instructions were highly efficacious (100% mortality) on EC-HL but differentially efficacious (62.2-84.4% mortality) on SF-HL examined eight days post-treatment. SF-HL that survived the first treatment received an identical second treatment eight days following the first treatment. Survivors (13.3-30.0%) developed to adults (10th-11th day following first treatment) and females successfully laid fertile eggs that developed to first instars. These results confirm resistance to permethrin- and pyrethrin-based pediculicide formulations in SF-HL when assessed under semi-clinical conditions and validates resistance previously determined using filter-paper contact bioassays and unformulated insecticides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 86, Issue 3, November 2006, Pages 195-202
Journal: Pesticide Biochemistry and Physiology - Volume 86, Issue 3, November 2006, Pages 195-202
نویسندگان
Kyong Sup Yoon, Joseph P. Strycharz, Jian-Rong Gao, Miwako Takano-Lee, John D. Edman, J. Marshall Clark,