Soil zymography - A novel in situ method for mapping distribution of enzyme activity in soil

► An in situ zymography technique for mapping and quantifying enzyme activity in soil was developed. ► The method allows exploring the two dimensional distribution of enzyme activities in soil. ► The distribution of protease and amylase activity in the rhizosphere of Lupinus polyphyllus was determined. ► Since the method works in situ it likely pictures enzyme activities more realistically than traditional enzyme assays.