کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2025875 | 1070012 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vertical advection of extracellular DNA by water capillarity in soil columns
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The fate of extracellular DNA in the environment concerns both the fate of transgenes from genetically modified organisms and the evolution of active bacteria capable of incorporating this DNA into their genomes. This study addressed the possibility that DNA, like other organic molecules, could move vertically in the capillary fringe of groundwater aquifers. The targeted gene fragment used here was the 35S-nptII sequence, which was below detection levels in controls. Initial microcosm studies detected the DNA target molecule by PCR during the entire experiment. The vertical advection of water and DNA were monitored for a period of 3 days in soil columns. DNA was added as a water solution at the bottom of the unsaturated soil column, and then DNA-free water was added at the bottom after 12 and 24Â h. After the addition of the DNA solution, capillary water rose 4Â cm within the soil column and the target DNA was detected up to that height. After 60Â min, the entire soil column (10Â cm) was wetted and the target sequence was detected up to a height of 7.5Â cm. After the second wetting (12Â h later), the target sequence was detected up to the top of the soil column (10Â cm). However, after the third wetting (24Â h later), the marker sequence was only found at heights from 0.5 to 4Â cm. Results clearly show the vertical movement of DNA due the capillary rise and suggest the possibility of DNA degradation within the soil column.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 39, Issue 1, January 2007, Pages 158-163
Journal: Soil Biology and Biochemistry - Volume 39, Issue 1, January 2007, Pages 158-163
نویسندگان
Maria Teresa Ceccherini, Judith Ascher, Giacomo Pietramellara, Timothy M. Vogel, Paolo Nannipieri,