Interactive effects of N fertilizer source and timing of fertilization leave specific N isotopic signatures in Chinese cabbage and soil

Natural 15N abundances (δ15N) in plant and soil can be used as a powerful marker to reveal the history of N fertilization. To investigate whether N fertilizer source and timing of fertilization leave specific δ15N signals in plant tissue and soil inorganic N, Chinese cabbage (Brassica campestris L. cv. Maeryok), one of the most popular vegetables in Asia, was grown in pots for 60 days with a single or split N applications of organic (composted manure; δ15N=+16.4‰) or inorganic N (urea; δ15N=−0.7‰). Seven N treatments were studied: (1) a single basal fertilization with compost or (2) urea; (3) a basal urea application followed by an additional (at 40 days after transplant, same below) compost or (4) urea application; (5) a basal compost application followed by an additional compost or (6) urea application; and (7) no N fertilization. Regardless of the time of N application, δ15N of cabbage treated with compost was higher (>+9.0‰) than that (< +1.0‰) treated with urea, reflecting the effect of isotopically different N sources. In split N fertilization, only the addition of isotopically different N sources in the middle of the growth period significantly affected the δ15N of the whole plant. Specific δ15N signals of basal N inputs were detected in outer cabbage parts formed in the early growth stage, while those of additional N inputs were detected in inner cabbage parts formed in the latter growth stage. We conclude that measurements of temporal variations in δ15N of plant parts formed in different growth stages could reveal the history of N fertilization.