کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2027494 1542693 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microbial transformation of contraceptive drug etonogestrel into new metabolites with Cunninghamella blakesleeana and Cunninghamella echinulata
ترجمه فارسی عنوان
تحول میکروبی etonogestrel داروی پیشگیری از بارداری به متابولیت های جدید با blakesleeana قارچ کانینگهام و echinulata قارچ کانینگهام
کلمات کلیدی
Etonogestrel؛ بارداری؛ زیست دگرگونی؛ Cunninghamella blakesleeana؛ Cunninghamella echinulata؛ مهار بتا گلوکورونیداز؛ سرطان کولون
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Five metabolites of contraceptive drug etonogestrel (1) were synthesized through biotransformation by fungi.
• Biotransformation of 1Cunninghamella blakesleeana yielded metabolites 2–6.
• Biotransformation of 1Cunninghamella echinulata yielded metabolites 2, 4, and 6.
• 6β-Hydroxy-etonogestrel (5) was found to be active against β-glucuronidase enzyme.

Biotransformation of a steroidal contraceptive drug, etonogestrel (1), (13-ethyl-17β-hydroxy-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-3-one) was investigated with Cunninghamella blakesleeana and C. echinulata. Five metabolites 2–6 were obtained on incubation of 1 with Cunninghamella blakesleeana, and three metabolites, 2, 4, and 6 were isolated from the transformation of 1 with C. echinulata. Among them, metabolites 2–4 were identified as new compounds. Their structures were deduced as 6β-hydroxy-11,22-epoxy-etonogestrel (2), 11,22-epoxy-etonogestrel (3), 10β-hydroxy-etonogestrel (4), 6β-hydroxy-etonogestrel (5), and 14α-hydroxy-etonogestrel (6). Compounds 1–6 were evaluated for various biological activities. Interestingly, compound 5 was found to be active against β-glucuronidase enzyme with IC50 value of 13.97 ± 0.12 μM, in comparison to standard compound, d-saccharic acid 1,4-lactone (IC50 = 45.75 ± 2.16 μM). Intestinal bacteria produce β-glucuronidase. Increased activity of β-glucuronidase is responsible for the hydrolyses of glucuronic acid conjugates of estrogen and other toxic substances in the colon, which plays a key role in the etiology of colon cancer. Inhibition of β-glucoronidase enzyme therefore has a therapeutic significance. Compounds 1–6 were also found to be non cytotoxic against 3T3 mouse fibroblast cell lines.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 115, November 2016, Pages 56–61
نویسندگان
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