Urinary OPN excretion in children with glomerular proteinuria

ABSTRACTPurposeThe aim of the study was to investigate urinary levels and clinical significance of osteopontin (uOPN) in children with different glomerular diseases according to histological diagnosis and degree of proteinuria.Materials and MethodsThe examinations were conducted in 3 groups of children: I - 20 children with minimal change disease (MCD) examined twice: A - in relapse; B – in remission, group II - 17 children with focal segmental glomerulosclerosis (FSGS), III - 12 children with IgA nephropathy (IgAN). The control group (C) contained 20 healthy children. OPN was measured in the urine using ELISA commercial available kit (R&D Quantikine) and was expressed in ng/ mg cr.ResultsThe median uOPN/ cr. in MCD children in relapse (IA) was median 134.98 ng/ mg cr. and was higher when compared to controls (p< 0.01). In exam IB, when proteinuria subsided, OPN/ cr. increased to median 172.96 ng/ mg cr. and was higher in comparison to healthy subjects (p< 0.01) and MCD children in relapse (p<0.05). Children from group II revealed higher uOPN/ cr. levels when compared to groups I, III and C (p< 0.01). UOPN/ cr. positively correlated with protein/ creatinine ratio in all examined groups of children (p< 0.01).ConclusionWe found significantly higher uOPN/ cr. in all the groups of children with glomerulonephritis. The highest uOPN/ cr. levels were found in patients with FSGS and correlated significantly with both interstitial changes and mesangial expansion found in kidney biopsy.