کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2032250 1071741 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microarray analysis reveals overexpression of IBSP in human carotid plaques
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Microarray analysis reveals overexpression of IBSP in human carotid plaques
چکیده انگلیسی

ABSTRACTPurposeVascular calcification was considered to be a passive, degenerative, and end-stage process of vascular disease. However, bone associated proteins such as bone morphogenetic proteins, osteopontin, osteonectin, osteocalcin, and matrix Gla protein (MGP) have been found in the calcified atherosclerotic lesions. We studied by microarray analysis whether intact tissue and carotid plaque from the same patient differ in transcriptional profiling in response to arterial calcification.Material and MethodsmRNA gene expression was measured by an Affymetrix GeneChip Human Gene 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) using RNA prepared from 68 specimens of endarterectomy from 34 patients.ResultsIntegrin-binding sialoprotein (IBSP) was found to be differentially expressed. IBSP mRNA is over expressed in atheroma plaque (3.74 fold, p = 1.41E-09) in an intraindividual comparison. Besides, Carbonic anhydrase II (CA2) which known to be a putative calcification inhibitory molecule is over expressed more than 1.7 fold in carotid plaque (p = 1.26E-06).ConclusionAlthough further evidence is needed, our results support previously available data. To our knowledge, this is the first report comparing gene expression between intact arterial tissue and carotid plaque using microarray analysis in order to identify calcification related genes. We suggest that plaques show a more pronounced induction of IBSP that may cause arterial calcification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Advances in Medical Sciences - Volume 57, Issue 2, December 2012, Pages 334–340
نویسندگان
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