کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2034542 | 1072026 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene–oligonucleotide conjugate
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene–oligonucleotide conjugate Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene–oligonucleotide conjugate](/preview/png/2034542.png)
چکیده انگلیسی
Novel biological activities of a fullerene–oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the β-actin cDNA was synthesized. We found that the FONC at a low concentration (10−6 M) could specifically inhibit PCR-amplification of the β-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 36, Issue 4, July 2008, Pages 223–226
Journal: Biologicals - Volume 36, Issue 4, July 2008, Pages 223–226
نویسندگان
Xinlin Yang, Xianmei Meng, Bo Li, Zhe Chen, Dongxu Zhao, Xin Tan, Qun Yu,