کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2034638 | 1072042 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries](/preview/png/2034638.png)
Different individuals, when infected with the same parasite, rarely produce antibodies against the same set of antigens. Indeed, unless a particular antigen happens to be recognized by antibodies in all individuals, the use of a single antigen in the serodiagnosis of parasitic diseases leads, invariably, to false-negative results. A straightforward method for pin-pointing, in genetic libraries, the precise antigens that would increase serodiagnostic assay sensitivities is presented. The method is based on the utilization of sera that produced false-negative results against previously available antigens. Employing this false-negative serum-selection methodology for the identification of new Leishmania infantum recombinant antigens (rAgs), the sensitivity of a dipstick assay for anti-Leishmania antibodies in a panel of sera from patients with visceral leishmaniasis was increased from 83.3% to 98.1%, without affecting its specificity, by the inclusion of a fifth and a sixth L. infantum rAg.
Journal: Biologicals - Volume 35, Issue 1, March 2007, Pages 51–54