کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2038952 1072998 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Western Zika Virus in Human Fetal Neural Progenitors Persists Long Term with Partial Cytopathic and Limited Immunogenic Effects
ترجمه فارسی عنوان
ویروس زایک غربی در پیش آگهی های عصبی جنین انسان پایدار طولانی مدت با اثرات منفی سیتوپاتیک و محدود
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• A 2015 ZIKV isolate replicates in human fetal neural progenitors
• ZIKV in fetal neural progenitors is partially cytopathic and persists for weeks
• ZIKV has limited immunogenic effects in fetal neural progenitors

SummaryThe recent Zika virus (ZIKV) outbreak in the Western hemisphere is associated with severe pathology in newborns, including microcephaly and brain damage. The mechanisms underlying these outcomes are under intense investigation. Here, we show that a 2015 ZIKV isolate replicates in multiple cell types, including primary human fetal neural progenitors (hNPs). In immortalized cells, ZIKV is cytopathic and grossly rearranges endoplasmic reticulum membranes similar to other flaviviruses. In hNPs, ZIKV infection has a partial cytopathic phase characterized by cell rounding, pyknosis, and activation of caspase 3. Despite notable cell death, ZIKV did not activate a cytokine response in hNPs. This lack of cell intrinsic immunity to ZIKV is consistent with our observation that virus replication persists in hNPs for at least 28 days. These findings, supported by published fetal neuropathology, establish a proof-of-concept that neural progenitors in the developing human fetus can be direct targets of detrimental ZIKV-induced pathology.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 11, 14 June 2016, Pages 2315–2322
نویسندگان
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