کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039051 1073017 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Subcellular Compartmentalization and Trafficking of the Biosynthetic Machinery for Fungal Melanin
ترجمه فارسی عنوان
جداسازی و قاچاق سلول های زیر سلولی دستگاه های بیوسنتز برای ملانین قارچی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Endosomal trafficking is critical for melanization in fungi
• There is stage-specific subcellular localization of the melanin biosynthetic enzymes
• Early melanin enzymes have no secretion signal and are atypical secretory proteins
• There is a unified cellular principle for melanogenesis in mammals and fungi

SummaryProtection by melanin depends on its subcellular location. Although most filamentous fungi synthesize melanin via a polyketide synthase pathway, where and how melanin biosynthesis occurs and how it is deposited as extracellular granules remain elusive. Using a forward genetic screen in the pathogen Aspergillus fumigatus, we find that mutations in an endosomal sorting nexin abolish melanin cell-wall deposition. We find that all enzymes involved in the early steps of melanin biosynthesis are recruited to endosomes through a non-conventional secretory pathway. In contrast, late melanin enzymes accumulate in the cell wall. Such subcellular compartmentalization of the melanin biosynthetic machinery occurs in both A. fumigatus and A. nidulans. Thus, fungal melanin biosynthesis appears to be initiated in endosomes with exocytosis leading to melanin extracellular deposition, much like the synthesis and trafficking of mammalian melanin in endosomally derived melanosomes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 11, 22 March 2016, Pages 2511–2518
نویسندگان
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