کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039115 1073027 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer
چکیده انگلیسی


• The conformation of the left arm of yeast chromosome III is mating type specific
• In MATa cells, HMLα is closer to the centromere and the MAT locus
• The recombination enhancer is a functionally composite element
• The right half of the RE is a cell-type-specific architectural element

SummaryMating-type switching in yeast occurs through gene conversion between the MAT locus and one of two silent loci (HML or HMR) on opposite ends of the chromosome. MATa cells choose HML as template, whereas MATα cells use HMR. The recombination enhancer (RE) located on the left arm regulates this process. One long-standing hypothesis is that switching is guided by mating-type-specific and possibly RE-dependent chromosome folding. Here, we use Hi-C, 5C, and live-cell imaging to characterize the conformation of chromosome III in both mating types. We discovered a mating-type-specific conformational difference in the left arm. Deletion of a 1-kb subregion within the RE, which is not necessary during switching, abolished mating-type-dependent chromosome folding. The RE is therefore a composite element with one subregion essential for donor selection during switching and a separate region involved in modulating chromosome conformation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 13, Issue 9, 1 December 2015, Pages 1855–1867
نویسندگان
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